|
KMID : 0357119930150020217
|
|
Korean Journal of Immunology
1993 Volume.15 No. 2 p.217 ~ p.225
|
|
|
Effects of Ascorbic Acid on the Phagocytosis of Murine Peritoneal Macrophages and the Activities of Lymphocytes
|
|
Lee Sang-Ik
Kang Young-Jun
Jeong Ga-Jin
|
|
|
Abstract
|
|
|
|
Ascorbic acid is the most important water soluble antioxidant as well as a cofactor in mangy hydroxylating reactions. There is a lot of evidence that immune system is sensitive to the level of ascorbic acid, and in our previous report it increased the antibody production of the mouse in vivo In this study we examined the effects of ascorbic acid on the phagocytosis and on the activities of the lymphocytes in mouse system. Murine peritoneal macrophages elicited with caseinate anc treated with ascorbic acid in vivo, showed increased phagocytic activity on the opsonized SRBC bui showed decreased detection of hydrogen peroxide(H202). When treated with ascorbic acid in vitro peritoneal macrophages showed no effect on phagocytosis. To examine the indirect effects of ascorbic acid on phagocytosis via lymphocytes, phorbol myristate acetate(PMA) -treated thymocy tes or T cells were incubated with ascorbic acid and macrophage activating capacity was assessed Culture supernatants of ascorbic acid-treated thymocytes and T cells enhanced the phagocytic activity of macrophages at the concentration of lug/ml. The proliferation of the thymocytes anc the blast formation of B cells were correlated with the level of ascorbic acid. Furthermore, ascorbic acid increased antibody production of splenocytes or B cells in vitro, confirming that it affect; immune system via lymphocytes. With the present results, we can explain in part the broad effect, of ascorbic acid on immune system by its influence on lymphocytes.
|
|
|
KEYWORD
|
|
|
Ascorbic acid, Phagocytosis, Lymphocytes
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|